Image Reconstruction

Data Post-Processing and Image Reconstruction

Basic Linux skills are required to use image reconstruction software. Access to GPU/CPU workstations are available on the 5^th floor of the Biochemistry building, room 502. The following data post-processing and image reconstruction software are available:

• RELION 3 (tutorial)
  • Requires GPU
• CisTEM (tutorial)
  • Only uses CPU
• SPHIRE (tutorial)
• EMAN2 (tutorial)
• PHENIX
  • An excellent resource for map analysis, sharpening, atomic model filling, etc.
• IMOD (tutorial)
  • The most popular software for tilt series data analysis and tomogram reconstruction

Additional compute resources are available at the MSU Institute for Cyber-Enabled Research (iCER).